Selective precipitation of phosphofructokinase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from rat erythrocyte hemolysates by polyethylene glycol.

The effect of copper on red cell enzyme activities.

Previous studies have shown a marked effect of very high levels of copper on red cell glucose-6-phosphate dehydrogenase and glutathione. When the effect of more nearly physiological levels of copper were studied, red cell hexokinase, phosphofructokinase, phosphoglyceric kinase, pyruvate kinase, and 6-phosphogluconate dehydrogenase were found to be inhibited. Inhibition was observed both when co...

Inhibition of Human Erythrocyte Glucose 6-Phosphate Dehydrogenase by Cadmium, Nickel and Aluminium

The effect of dietary bagasse on the activities of some key enzymes of carbohydrate and lipid metabolism in mouse liver.

The effects of a 100 g/kg diet substitution of bagasse on the body-weight gain, food consumption and faecal dry weight of mice given a high-sucrose diet and on the activities of hepatic glucose-6-phosphate dehydrogenase (EC I.I.I.49), 6-phosphogluconate dehydrogenase (EC I.I.I.44), malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC I.I.I.40), ATP-citrate (pro-3S) lyase (EC 4.I.3.8)...

Inhibition of rat liver glucose-6-phosphate dehydrogenase synthesis by glucagon.

In fasting-refeeding experiments glucagon was found to inhibit the induction of rat liver glucose-6-P dehydrogenase. The kinetics of induction in the presence of glucagon indicated that the hormone decreased the rate of enzyme synthesis without altering the rate of enzyme degradation. Immunochemical titration of the amount of glucose-6-P dehydrogenase protein present in control and glucagon tre...

Measurement of erythrocyte glucose-6-phosphate dehydrogenase activity using a centrifugal analyzer.

We describe two improved methods for determination of erythrocyte glucose-6-phosphate dehydrogenase activity. One method is an “enzyme-linked” procedure in which an excess of 6-phosphogluconate dehydrogenase is used to produce two moles of NADPHfor each mole of glucose-6-phosphate oxidized. In the other method 2,3-diphosphoglycerate is used to inhibit the variable contribution of endogenous 6-p...